PT - JOURNAL ARTICLE AU - ANA MARGARIDA MADUREIRA AU - GABRIELLA SPENGLER AU - ANNAMARIA MOLNAR AU - ANDREAS VARGA AU - JOSEPH MOLNAR AU - PEDRO M. ABREU AU - MARIA-JOSÉ U. FERREIRA TI - Effect of Cycloartanes on Reversal of Multidrug Resistance and Apoptosis Induction on Mouse Lymphoma Cells DP - 2004 Mar 01 TA - Anticancer Research PG - 859--864 VI - 24 IP - 2B 4099 - http://ar.iiarjournals.org/content/24/2B/859.short 4100 - http://ar.iiarjournals.org/content/24/2B/859.full SO - Anticancer Res2004 Mar 01; 24 AB - The ability of fifteen cycloartanes, isolated from Euphorbia species, to reverse multidrug resistance (MDR) and apoptosis induction in L5178Y mouse lymphoma cells, including its multidrug-resistant subline, was studied by flow cytometry. Reversion of MDR was investigated using a standard functional assay with rhodamine 123 as a fluorescent substrate analogue. For the evaluation of apoptosis, the cells were stained with FITC-labeled annexin V and propidium iodide. The majority of the compounds were able to reverse MDR of the tested human MDR1 gene-transfected mouse lymphoma cells. Some of the compounds were able to induce moderate apoptosis in the PAR cell line, but this effect was less effective on multidrug-resistant cells. The results indicate that cycloartanes can be substrates of ABC transporters, which might compete with certain anticancer chemotherapeutics. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved