RT Journal Article SR Electronic T1 Increased Nuclear Factor-kB Activation in Human Colorectal Carcinoma and its Correlation with Tumor Progression JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 675 OP 682 VO 24 IS 2B A1 MASAYUKI KOJIMA A1 TAKASHI MORISAKI A1 NOBUHIKO SASAKI A1 KENJI NAKANO A1 RYUICHI MIBU A1 MASAO TANAKA A1 MITSUO KATANO YR 2004 UL http://ar.iiarjournals.org/content/24/2B/675.abstract AB Objectives: Nuclear factor-kB (NF-kB) is a transcription factor that participates in the induction of several genes for cytokines and enzymes that play important functional roles in various cell types. The aim of this study was to determine NF-kB activation status in human colorectal carcinoma and its correlation to the clinicopathological characteristics of patients. Materials and Methods: We examined the activation status of NF-kB in 28 resected colorectal carcinomas and in colonic mucosa from uninvolved portions of these specimens by electrophoretic mobility shift assay (EMSA) and immunohistochemical staining for the p65 subunit of NF-kB. Results: EMSA showed much greater activation in the tumors than in normal mucosa, as did epithelial p65 immunostaining. NF-kB activation significantly increased in the more progressed cases (T3+T4 cases or Stage II< cases). In vitro studies using lipopolysaccharide (LPS)-responsive colon carcinoma cells suggested a correlation between NF-kB activation and cell proliferation. Conclusion: Our findings indicated that NF-kB is constitutively activated in human colorectal carcinoma tissue and correlates with tumor progression. The regulation of this transcription factor might be therapeutically useful against these tumors. Copyright© 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved