%0 Journal Article %A PAOLO MARCHETTI %A DOMENICO A.P. GALLA %A F. PAOLA RUSSO %A ENRICO RICEVUTO %A VINCENZO FLATI %A GIAMPIERO PORZIO %A CORRADO FICORELLA %A M. GRAZIA CIFONE %T Apoptosis Induced by Oxaliplatin in Human Colon Cancer HCT15 Cell Line %D 2004 %J Anticancer Research %P 219-226 %V 24 %N 1 %X Background: Oxaliplatin (L-OHP), active in a wide range of human and animal tumours, also CDDP-resistant, possesses unique molecular characteristics of action. However, the mechanisms by which the damage induced by L-OHP triggers a death signal are not yet fully defined. Materials and Methods: After L-OHP treatment of the HCT15 human colon cancer cell line, apoptosis was evaluated by DNA laddering detection and by flow cytometry; the effect on specific caspase-3, -8 and -9 inhibitors, mitochondrial membrane permeability transition, cytochrome C release and expression of CD95 and CD95L were also assessed. Results: HCT15 cells underwent apoptosis when treated with all used drug concentrations (7-25 μM). Treatment of cells with L-OHP resulted in the activation of caspase-8, -9 and -3, in a mitochondrial membrane depolarisation, and in an increase of CD95 receptor and CD95 ligand levels. Conclusion: The results correlated well with the ability of L-OHP to induce apoptosis and give further insights into the mechanisms underlying the L-OHP-induced apoptosis of tumor cells. %U https://ar.iiarjournals.org/content/anticanres/24/1/219.full.pdf