PT  - JOURNAL ARTICLE
AU  - KIMBERLY A. CHAPMAN
AU  - ERCOLE L. CAVALIERI
AU  - ELEANOR G. ROGAN
TI  - Repair of Short Oligodeoxyribonucleotides Containing a Stable Adduct and an Apurinic Site by Extracts of MCF-10A1 Cells
DP  - 2004 Jan 01
TA  - Anticancer Research
PG  - 117--122
VI  - 24
IP  - 1
4099  - http://ar.iiarjournals.org/content/24/1/117.short
4100  - http://ar.iiarjournals.org/content/24/1/117.full
SO  - Anticancer Res2004 Jan 01; 24
AB  - Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions.