TY - JOUR T1 - Repair of Short Oligodeoxyribonucleotides Containing a Stable Adduct and an Apurinic Site by Extracts of MCF-10A1 Cells JF - Anticancer Research JO - Anticancer Res SP - 117 LP - 122 VL - 24 IS - 1 AU - KIMBERLY A. CHAPMAN AU - ERCOLE L. CAVALIERI AU - ELEANOR G. ROGAN Y1 - 2004/01/01 UR - http://ar.iiarjournals.org/content/24/1/117.abstract N2 - Background: DNA with two sites of damage in close proximity might not be repaired as efficiently as DNA with a single damage site. Materials and Methods: To study this hypothesis, we utilized short oligodeoxyribonucleotides with a stable adduct 7 or 16 nucleotides (nt) downstream from an apurinic (AP) site. Repair by extracts of human breast epithelial MCF-10A1 cells was assayed by quantifying the incorporation of [α-32P]dTTP. Results: The level of repair of an oligodeoxyribonucleotide with an AP site 7 nt from a stable adduct was comparable to that of the oligodeoxyribonucleotide with only an AP site. A decrease in overall repair of oligodeoxyribonucleotides containing an AP site and a stable adduct was observed if these lesions were 16 nt apart compared to the presence of only an AP site. Conclusion: The ability of human breast MCF-10A1 cells to repair DNA adducts and AP sites is affected by other near-by lesions. ER -