RT Journal Article
SR Electronic
T1 18β-Glycyrrhetinic Acid and Glabridin Prevent Oxidative DNA Fragmentation in UVB-irradiated Human Keratinocyte Cultures
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2209
OP 2215
VO 31
IS 6
A1 VERATTI, EUGENIA
A1 ROSSI, TIZIANA
A1 GIUDICE, STEFANIA
A1 BENASSI, LUISA
A1 BERTAZZONI, GIORGIA
A1 MORINI, DARIA
A1 AZZONI, PAOLA
A1 BRUNI, ELISA
A1 GIANNETTI, ALBERTO
A1 MAGNONI, CRISTINA
YR 2011
UL http://ar.iiarjournals.org/content/31/6/2209.abstract
AB Background: UVB radiation is the major etiological factor in the pathogenesis of skin aging and cancer development. New approaches to prevent and reverse UVB damage are needed to reduce sunlight-induced skin cancer. This study aimed to investigate a possible protective activity of liquorice root extracts glycyrrhizin (GL), 18β-glycyrrhetinic acid (18β-GA) and glabridin (GLB) against UVB radiation damage in human keratinocyte cultures. Materials and Methods: The MTT test was performed to assess cell viability. DNA damage was evaluated by comet assay, whereas generation of intracellular reactive oxygen species (ROS) was measured by fluorescent 2'7'-dichlorodihydrofluorescein diacetate assay. In addition, the activation of p53, regulation of BCL-2 and PARP cleavage were analyzed by Western blot analysis. Results: The treatment of human keratinocytes with 18β-GA and GLB prevented direct and indirect DNA damage avoiding apoptosis activation. Conclusion: 18β-glycyrrhetinic acid and glabridin are potent antioxidants that prevent oxidative DNA fragmentation and the activation of apoptosis-associated proteins in human keratinocytes.