PT  - JOURNAL ARTICLE
AU  - EUGENIA VERATTI
AU  - TIZIANA ROSSI
AU  - STEFANIA GIUDICE
AU  - LUISA BENASSI
AU  - GIORGIA BERTAZZONI
AU  - DARIA MORINI
AU  - PAOLA AZZONI
AU  - ELISA BRUNI
AU  - ALBERTO GIANNETTI
AU  - CRISTINA MAGNONI
TI  - 18β-Glycyrrhetinic Acid and Glabridin Prevent Oxidative DNA Fragmentation in UVB-irradiated Human Keratinocyte Cultures
DP  - 2011 Jun 01
TA  - Anticancer Research
PG  - 2209--2215
VI  - 31
IP  - 6
4099  - http://ar.iiarjournals.org/content/31/6/2209.short
4100  - http://ar.iiarjournals.org/content/31/6/2209.full
SO  - Anticancer Res2011 Jun 01; 31
AB  - Background: UVB radiation is the major etiological factor in the pathogenesis of skin aging and cancer development. New approaches to prevent and reverse UVB damage are needed to reduce sunlight-induced skin cancer. This study aimed to investigate a possible protective activity of liquorice root extracts glycyrrhizin (GL), 18β-glycyrrhetinic acid (18β-GA) and glabridin (GLB) against UVB radiation damage in human keratinocyte cultures. Materials and Methods: The MTT test was performed to assess cell viability. DNA damage was evaluated by comet assay, whereas generation of intracellular reactive oxygen species (ROS) was measured by fluorescent 2'7'-dichlorodihydrofluorescein diacetate assay. In addition, the activation of p53, regulation of BCL-2 and PARP cleavage were analyzed by Western blot analysis. Results: The treatment of human keratinocytes with 18β-GA and GLB prevented direct and indirect DNA damage avoiding apoptosis activation. Conclusion: 18β-glycyrrhetinic acid and glabridin are potent antioxidants that prevent oxidative DNA fragmentation and the activation of apoptosis-associated proteins in human keratinocytes.