<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">VERATTI, EUGENIA</style></author><author><style face="normal" font="default" size="100%">ROSSI, TIZIANA</style></author><author><style face="normal" font="default" size="100%">GIUDICE, STEFANIA</style></author><author><style face="normal" font="default" size="100%">BENASSI, LUISA</style></author><author><style face="normal" font="default" size="100%">BERTAZZONI, GIORGIA</style></author><author><style face="normal" font="default" size="100%">MORINI, DARIA</style></author><author><style face="normal" font="default" size="100%">AZZONI, PAOLA</style></author><author><style face="normal" font="default" size="100%">BRUNI, ELISA</style></author><author><style face="normal" font="default" size="100%">GIANNETTI, ALBERTO</style></author><author><style face="normal" font="default" size="100%">MAGNONI, CRISTINA</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">18β-Glycyrrhetinic Acid and Glabridin Prevent Oxidative DNA Fragmentation in UVB-irradiated Human Keratinocyte Cultures</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2011</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2011-06-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">2209-2215</style></pages><volume><style face="normal" font="default" size="100%">31</style></volume><issue><style face="normal" font="default" size="100%">6</style></issue><abstract><style  face="normal" font="default" size="100%">Background: UVB radiation is the major etiological factor in the pathogenesis of skin aging and cancer development. New approaches to prevent and reverse UVB damage are needed to reduce sunlight-induced skin cancer. This study aimed to investigate a possible protective activity of liquorice root extracts glycyrrhizin (GL), 18β-glycyrrhetinic acid (18β-GA) and glabridin (GLB) against UVB radiation damage in human keratinocyte cultures. Materials and Methods: The MTT test was performed to assess cell viability. DNA damage was evaluated by comet assay, whereas generation of intracellular reactive oxygen species (ROS) was measured by fluorescent 2'7'-dichlorodihydrofluorescein diacetate assay. In addition, the activation of p53, regulation of BCL-2 and PARP cleavage were analyzed by Western blot analysis. Results: The treatment of human keratinocytes with 18β-GA and GLB prevented direct and indirect DNA damage avoiding apoptosis activation. Conclusion: 18β-glycyrrhetinic acid and glabridin are potent antioxidants that prevent oxidative DNA fragmentation and the activation of apoptosis-associated proteins in human keratinocytes.</style></abstract></record></records></xml>