PT  - JOURNAL ARTICLE
AU  - TOMOKO KANEKO
AU  - MASAHIDE TOMINAGA
AU  - RISA KOUZAKI
AU  - AYAKA HANYU
AU  - KAZUKI UESHIMA
AU  - HISATSUGU YAMADA
AU  - MASAKI SUGA
AU  - TOMOHIRO YAMASHITA
AU  - TOMOAKI OKIMOTO
AU  - YOSHIHIRO UTO
TI  - Radiosensitizing Effect of 5-Aminolevulinic Acid and Protoporphyrin IX on Carbon-ion Beam Irradiation
AID  - 10.21873/anticanres.12730
DP  - 2018 Jul 01
TA  - Anticancer Research
PG  - 4313--4317
VI  - 38
IP  - 7
4099  - http://ar.iiarjournals.org/content/38/7/4313.short
4100  - http://ar.iiarjournals.org/content/38/7/4313.full
SO  - Anticancer Res2018 Jul 01; 38
AB  - Background/Aim: Carbon-ion beam is one of the most advanced radiations used for cancer treatment. However, there are tumors that are difficult to suppress with carbon-ion beam alone, thus necessitating development of drugs that can enhance its therapeutic effect. In this regard, the radiosensitizing effect of 5-aminolevulinic acid (ALA) and protoporphyrin IX (PpIX), that is a metabolic intermediate of ALA, on carbon-ion beam was investigated. Materials and Methods: Radiosensitizing activity, mitochondrial ROS and DNA double-strand break production of ALA and PpIX were evaluated by irradiation with 1.0 or 1.5-Gy carbon-ion beam to mouse mammary EMT6 tumor cells. Results: Combination of carbon-ion beam and ALA or PpIX showed a significant enhancement of its cytotoxic activity through a significant increase in ROS production in mitochondria. Furthermore, the combined activity of carbon-ion beam and ALA resulted in a significant increase in DNA double-strand breakage. Conclusion: ALA selectively accumulates in the mitochondria and PpIX synthesized from ALA reacts with carbon-ion beam to produce ROS that exert antitumor activity.