@article {MURRAY489, author = {DAVID MURRAY and MARY MORRIN and SUSAN MCDONNELL}, title = {Increased Invasion and Expression of MMP-9 in Human Colorectal Cell Lines by a CD44-dependent Mechanism}, volume = {24}, number = {2A}, pages = {489--494}, year = {2004}, publisher = {International Institute of Anticancer Research}, abstract = {Background: In order to investigate the interactions between MMPs and CD44 we stably transfected a non-invasive colon cell line, SW480 with the cDNA for MMP-9 and investigated the effect on CD44 expression and in vitro invasion and migration. Materials and Methods: In vitro invasion and migration assays were carried out using Biocoat matrigel invasion chambers. MMP and CD44 expression was determined using zymography, Western blot analysis and RTPCR. Results: Transfection of the parental SW480 cells with the cDNA for MMP-9 (SW480M9) caused increased invasion and migration. MMP-9 expression increased when the SW480M9 cells were grown on HA and collagen and cultured in the presence of a CD44-activating antibody. Treatment of the cells with HA and a CD44-activating antibody also resulted in increased invasion, migration and attachment. Conclusion: These results demonstrated that CD44 and MMP interactions are important in controlling tumour cell invasion and migration. Copyright{\textcopyright} 2004 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/24/2A/489}, eprint = {https://ar.iiarjournals.org/content/24/2A/489.full.pdf}, journal = {Anticancer Research} }