RT Journal Article
SR Electronic
T1 Huachansu, Containing Cardiac Glycosides, Enhances Radiosensitivity of Human Lung Cancer Cells
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 2141
OP 2148
VO 31
IS 6
A1 LI WANG
A1 UMA RAJU
A1 LUKA MILAS
A1 DAVID MOLKENTINE
A1 ZHEN ZHANG
A1 PEIYING YANG
A1 LORENZO COHEN
A1 ZHIQIANG MENG
A1 ZHONGXING LIAO
YR 2011
UL http://ar.iiarjournals.org/content/31/6/2141.abstract
AB Aim: To assess radiosensitzing potential of huachansu (HCS) and delineate the underlying mechanisms. Materials and Methods: Lung cancer cell lines were exposed to HCS, radiation or both and subjected to survival assays, Western blots, apoptosis assay and immunocytochemical analysis. Results: HCS suppressed the viability of all three lung lines tested and enhanced radiosensitivity of H460 and A549 (wild-type p53) only with no effect on H1299 (p53 null) cells. HCS prolonged the presence of radiation-induced γH2AX foci and increased radiation-induced apoptosis. Western blots showed that HCS increased cleaved caspase-3 and cleaved poly-(ADP-ribose) polymerase (PARP) levels, as well as reducing BCL-2 and p53 protein levels in H460 cells. Conclusion: HCS-enhanced radiosensitivity of human lung cancer lines appeared to be p53-dependent. Inhibition of DNA repair and increase in radiation-induced apoptosis may have served as underlying mechanisms. These data suggest that HCS may have potential to improve the efficacy of radiotherapy.