PT - JOURNAL ARTICLE AU - DAISUKE UEJIMA AU - KOICHI NISHIJO AU - YOICHIRO KAJITA AU - TATSUYA ISHIBE AU - TOMOKI AOYAMA AU - SUSUMU KAGEYAMA AU - HIDEAKI IWAKI AU - TAKASHI NAKAMURA AU - HIROKAZU IIDA AU - TATSUHIRO YOSHIKI AU - JUNYA TOGUCHIDA TI - Involvement of Cancer Biomarker C7orf24 in the Growth of Human Osteosarcoma DP - 2011 Apr 01 TA - Anticancer Research PG - 1297--1305 VI - 31 IP - 4 4099 - http://ar.iiarjournals.org/content/31/4/1297.short 4100 - http://ar.iiarjournals.org/content/31/4/1297.full SO - Anticancer Res2011 Apr 01; 31 AB - Background: Up-regulation of the expression of the gene C7orf24, encoding γ-glutamyl cyclotransferase, is a common event in cancers derived from various tissues, but its involvement in osteosarcomas (OS) has not yet been demonstrated. Materials and Methods: The expression of C7orf24 was analyzed in human OS cell lines and primary tumor samples. The biological effects of C7orf24 on growth, motility, and invasion in the OS cell lines were investigated using siRNA for C7orf24. Genes related to the function of C7orf24 were sought by genome-wide gene expression profiling. Results: The level of C7orf24 expression was much higher in the OS cell lines and OS primary tumors than in normal osteoblasts. Down-regulation of C7orf24 expression inhibited the growth of the cell lines in association with enhancement of cell-clustering. Treatment with C7orf24-siRNA inhibited cell motility and invasion. Gene ontology suggested the function of C7orf24 to be related to cell adhesion and protein transport. Conclusion: C7orf24 is also involved in the growth of OS, and is a potential biomarker for this type of tumor.