PT  - JOURNAL ARTICLE
AU  - KITSADA KANGBOONRUANG
AU  - PATOMPON WONGTRAKOONGATE
AU  - KORNKAMON LERTSUWAN
AU  - SUPHALAK KHACHONKHAM
AU  - PIMOLPUN CHANGKAEW
AU  - PUANGPEN TANGBOONDUANGJIT
AU  - TEERADA SIRIPOON
AU  - NUTTAPONG NGAMPHAIBOON
AU  - ARTHIT CHAIROUNGDUA
TI  - <em>MALAT1</em> Decreases the Sensitivity of Head and Neck Squamous Cell Carcinoma Cells to Radiation and Cisplatin
AID  - 10.21873/anticanres.14235
DP  - 2020 May 01
TA  - Anticancer Research
PG  - 2645--2655
VI  - 40
IP  - 5
4099  - http://ar.iiarjournals.org/content/40/5/2645.short
4100  - http://ar.iiarjournals.org/content/40/5/2645.full
SO  - Anticancer Res2020 May 01; 40
AB  - Background/Aim: Two-thirds of head and neck squamous cell carcinoma (HNSCC) patients present with locally advanced (LA) stages and have a poor survival rate. The aim of this study was to investigate the roles of the long non-coding RNAs MALAT1 on radiation and cisplatin sensitivity of HNSCC cells. Materials and Methods: Clonogenic, cell viability, and apoptosis assays were performed in cells following MALAT1 knockdown using CRISPR/Cas9 system. Results: MALAT1 was overexpressed in HNSCC cell lines as compared to a non-tumorigenic cell line. The number of colonies formed after radiation was significantly reduced in MALAT1 knockdown cells. The IC50 value of cisplatin in MALAT1 knockdown cells was lower than that of the control cells. MALAT1 knockdown resulted in cell cycle arrest at G2/M phase, DNA damage and apoptotic cell death. Conclusion: MALAT1 knockdown enhanced the sensitivity of HNSCC cells to radiation and cisplatin partly through the induction of G2/M cell cycle arrest resulting in DNA damage and apoptosis.