RT Journal Article SR Electronic T1 Oropharyngeal HPV Detection Techniques in HPV-associated Head and Neck Cancer Patients JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 2117 OP 2123 DO 10.21873/anticanres.14170 VO 40 IS 4 A1 TANJA K. EGGERSMANN A1 PHILIPP BAUMEISTER A1 JORG KUMBRINK A1 DORIS MAYR A1 ELISA SCHMOECKEL A1 CHRISTIAN J. THALER A1 CHRISTIAN DANNECKER A1 UDO JESCHKE A1 THOMAS NAGLER A1 SVEN MAHNER A1 KARIEM SHARAF A1 JULIA K. S. GALLWAS YR 2020 UL http://ar.iiarjournals.org/content/40/4/2117.abstract AB Background/Aim: The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention. Patients and Methods: Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue. Results: Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods. Conclusion: Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended.