TY - JOUR T1 - Oropharyngeal HPV Detection Techniques in HPV-associated Head and Neck Cancer Patients JF - Anticancer Research JO - Anticancer Res SP - 2117 LP - 2123 DO - 10.21873/anticanres.14170 VL - 40 IS - 4 AU - TANJA K. EGGERSMANN AU - PHILIPP BAUMEISTER AU - JORG KUMBRINK AU - DORIS MAYR AU - ELISA SCHMOECKEL AU - CHRISTIAN J. THALER AU - CHRISTIAN DANNECKER AU - UDO JESCHKE AU - THOMAS NAGLER AU - SVEN MAHNER AU - KARIEM SHARAF AU - JULIA K. S. GALLWAS Y1 - 2020/04/01 UR - http://ar.iiarjournals.org/content/40/4/2117.abstract N2 - Background/Aim: The incidence of human papilloma virus (HPV)-related head and neck squamous cell carcinoma (HNSCC) has been increasing in the last decades. Analysis of oral brushing or rinsing samples for screening or stratification could potentially improve screening and prevention. Patients and Methods: Oral brushes and mouthwashes were taken from 20 patients with HPV-associated HNSCC before definite therapy. HPV genotyping was performed for the detection of 14 high-risk HPV subtypes and correlated to DNA isolated from tumor tissue. Results: Ten of 20 patients were tested HPV positive by using either method. There was a significant correlation between macroscopic visibility of tumor and positive HPV detection (p<0.001) and HPV detection and tumor size (p<0.001). HPV was detected in all macroscopically visible tumors. Half of the HPV cases who had macroscopically invisible tumors were missed by both methods. Conclusion: Both techniques are limited in the detection of macroscopically non-visible and small tumors. Therefore, the application of these techniques for screening or diagnosis of HNSCC is not recommended. ER -