PT  - JOURNAL ARTICLE
AU  - SEMO JUN
AU  - SEOK WON KIM
AU  - JI-YEON LIM
AU  - SEON-JOO PARK
TI  - ABHD12 Knockdown Suppresses Breast Cancer Cell Proliferation, Migration and Invasion
AID  - 10.21873/anticanres.14231
DP  - 2020 May 01
TA  - Anticancer Research
PG  - 2601--2611
VI  - 40
IP  - 5
4099  - http://ar.iiarjournals.org/content/40/5/2601.short
4100  - http://ar.iiarjournals.org/content/40/5/2601.full
SO  - Anticancer Res2020 May 01; 40
AB  - Background/Aim: Alpha/beta-hydrolase domain containing 12 (ABHD12) is a serine hydrolase that regulates immunological and neurological mechanisms. This study aimed to elucidate the oncogenic effect of ABHD12 on human breast cancer. Materials and Methods: ABHD12 expression was confirmed in breast cancer tissues and breast cancer cell lines by immunohistochemistry and quantitative RT-PCR. To determine the role of ABHD12, ABHD12 siRNA-suppressed breast cancer cells (MCF7 and MDA-MB-231 cells) were investigated for cell proliferation, migration, and invasion capabilities using MTT assays, EdU assays, colony formation assays, and Boyden chamber assays. Results: Immunohistochemical staining showed a higher ABHD12 expression in breast cancer tissues than in normal tissues. Additionally, ABHD12 knockdown was found to inhibit cell growth, proliferation, migration, and invasion in breast cancer cells. Conclusion: ABHD12 plays a crucial role in cell proliferation, migration, and invasion of breast cancer cells.