PT  - JOURNAL ARTICLE
AU  - TAE-GUL LEE
AU  - SEO YUN KIM
AU  - HYE-RYOUN KIM
AU  - HYUNGGEE KIM
AU  - CHEOL HYEON KIM
TI  - Radiation Induces Autophagy <em>via</em> Histone H4 Lysine 20 Trimethylation in Non-small Cell Lung Cancer Cells
AID  - 10.21873/anticanres.14224
DP  - 2020 May 01
TA  - Anticancer Research
PG  - 2537--2548
VI  - 40
IP  - 5
4099  - http://ar.iiarjournals.org/content/40/5/2537.short
4100  - http://ar.iiarjournals.org/content/40/5/2537.full
SO  - Anticancer Res2020 May 01; 40
AB  - Background/Aim: Radiotherapy-induced autophagy affects radiation-sensitivity and radiotherapy efficacy. Histone modifications also occur during radiotherapy. This study assessed radiotherapy effects on histone modification and autophagy in non-small cell lung cancer (NSCLC) cells. Materials and Methods: NSCLC cells were subjected to γ-irradiation. Autophagy was detected using western blotting and acridine orange staining. Radiation effect on cell growth was evaluated by clonogenic assay. Histone modifications were assessed by western blotting. Next generation sequencings (NGSs) were conducted to identify histone modification target genes. Results: Radio-protective autophagy and histone H4 lysine 20 trimethylation (H4K20me3) were up-regulated after irradiation. By NGSs, genes that are differentially expressed upon irradiation were identified, including the candidate H4K20me3 target gene GABARAPL1. Furthermore, we showed that GABARAPL1 is essential for the radiation-induced autophagy. Conclusion: Our findings revealed the regulatory axis of radiation-induced H4K20me3-GABARAPL1 in radio-protective autophagy. Modulation of this axis may be a new strategy to enhance radiotherapy efficacy in NSCLC.