TY - JOUR T1 - Glioblastoma Factors Increase the Migration of Human Brain Endothelial Cells <em>In Vitro</em> by Increasing MMP-9/CXCR4 Levels JF - Anticancer Research JO - Anticancer Res SP - 2725 LP - 2737 DO - 10.21873/anticanres.14244 VL - 40 IS - 5 AU - LUCIANE VIEIRA DE OLIVEIRA ROSARIO AU - BARBARA GOMES DA ROSA AU - THAYNAN LOPES GONCALVES AU - DIANA ISABEL LOURENCO MATIAS AU - CATARINA FREITAS AU - VALERIA PEREIRA FERRER Y1 - 2020/05/01 UR - http://ar.iiarjournals.org/content/40/5/2725.abstract N2 - Background/Aim: Glioblastoma (GB) is the most aggressive type of tumor in the central nervous system and is characterized by resistance to therapy and abundant vasculature. Tumor vessels contribute to the growth of GB, and the tumor microenvironment is thought to influence tumor vessels. We evaluated the molecular communication between human GB cells and human brain microvascular endothelial cells (HBMEC) in vitro. Materials and Methods: We investigated whether GB-conditioned media (GB-CM) influenced HBMEC proliferation and migration, as well as the levels of MMP-9, CXCL12, CXCR4, CXCR7, VEGFs, VEGFR-2, and WNT5a in HBMEC. Results: Although HBMEC proliferation was not modified, increased HBMEC migration was detected after GB-CM treatment. Furthermore, treatment of HBMEC with GB-CM resulted in increased levels of MMP-9 and CXCR4. The levels of WNT5a, VEGFs and VEGFR-2 were not affected. Conclusion: GB-secreted factors lead to increased endothelial cell migration and to increased levels of MMP-9 and CXCR4. ER -