%0 Journal Article %A KENTARO IGARASHI %A KEI KAWAGUCHI %A MING ZHAO %A QINGHONG HAN %A YUYING TAN %A TASUKU KIYUNA %A KENTARO MIYAKE %A TAKASHI HIGUCHI %A SCOTT D. NELSON %A SARAH M. DRY %A YUNFENG LI %A NORIO YAMAMOTO %A KATSUHIRO HAYASHI %A HIROAKI KIMURA %A SHINJI MIWA %A SHREE RAM SINGH %A HIROYUKI TSUCHIYA %A ROBERT M. HOFFMAN %T Recombinant Methioninase Combined With Tumor-targeting Salmonella typhimurium A1-R Induced Regression in a PDOX Mouse Model of Doxorubicin-resistant Dedifferentiated Liposarcoma %D 2020 %R 10.21873/anticanres.14222 %J Anticancer Research %P 2515-2523 %V 40 %N 5 %X Background/Aim: Dedifferentiated liposarcoma (DDLPS) is associated with a poor survival rate even with multi-modality treatment. In the present study, we evaluated the efficacy of recombinant methioninase (rMETase) combined with tumor-targeting Salmonella typhimurium (S. typhimurium) A1-R against a doxorubicin-resistant DDLPS in a patient-derived orthotopic xenograft (PDOX) mouse model. Materials and Methods: A recurrent high-grade DDLPS from the right retroperitoneum of a patient was grown orthotopically in the retroperitoneum of nude mice to establish a PDOX model. The PDOX models were randomly divided into the following groups: Control, no treatment; doxorubicin monotherapy; rMETase monotherapy; S. typhimurium A1-R monotherapy; S. typhimurium A1-R and rMETase combination therapy. Tumor length and width were measured before and after treatment. Results: On day 14 after treatment, all treatments significantly inhibited DDLPS PDOX tumor growth compared to the untreated control except for doxorubicin monotherapy. rMETase combined with S. typhimurium A1-R was significantly more effective and regressed tumor volume compared to either rMETase or S. typhimurium A1-R alone. The relative body weight did not significantly differ between days 0 and 14 for individual groups. Conclusion: The combination of rMETase and S. typhimurium A1-R has important clinical potential for this recalcitrant sarcoma. %U https://ar.iiarjournals.org/content/anticanres/40/5/2515.full.pdf