PT  - JOURNAL ARTICLE
AU  - SRIRATANASAK, NICHARAT
AU  - NONPANYA, NONGYAO
AU  - WATTANATHANA, WORAWAT
AU  - CHANVORACHOTE, PITHI
TI  - Benzoxazine Dimer Analogue Targets Integrin β3 in Lung Cancer Cells and Suppresses Anoikis Resistance and Migration
AID  - 10.21873/anticanres.14229
DP  - 2020 May 01
TA  - Anticancer Research
PG  - 2583--2589
VI  - 40
IP  - 5
4099  - http://ar.iiarjournals.org/content/40/5/2583.short
4100  - http://ar.iiarjournals.org/content/40/5/2583.full
SO  - Anticancer Res2020 May 01; 40
AB  - Background/Aim: Certain integrins including integrin β3 facilitate movement and survival of metastatic cancer cells. We examined whether benzoxazine dimer analogue N,N-bis(5-ethyl-2-hydroxybenzyl) methylamine (HM) has anti-metastatic effects. Materials and Methods: Cell viability was examined by the 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) assay. Wound healing and phalloidin-rhodamine assays were performed to evaluate the migration and filopodia formation, respectively. Anoikis resistance was studied by anchorage-independent growth assay. The expression of proteins regulating migration were examined by western blot. Results: HM treatment significantly inhibited growth and survival of detached lung cancer cells as indicated by the reduced colony number and size of anchorage-independent growth analysis. HM inhibited cell migration and suppressed filopodia formation. Protein analysis indicated that the compound dramatically decreased integrin β3 and its related downstream proteins including active focal adhesion kinase (FAK) and active protein kinase B (AKT); however, integrin β1 and α5 were found to be unaltered. Conclusion: HM shows a potential in targeting integrin β3 and could be a good candidate for further developed as an anti-metastatic therapy.