PT  - JOURNAL ARTICLE
AU  - RANIA ALAAELDIN
AU  - MAIIADA HASSAN NAZMY
AU  - MOHAMED ABDEL-AZIZ
AU  - GAMAL EL-DIN A. ABUO-RAHMA
AU  - MOUSTAFA FATHY
TI  - Cell Cycle Arrest and Apoptotic Effect of 7-(4-(N-substituted carbamoylmethyl) piperazin-1-yl) Ciprofloxacin-derivative on HCT 116 and A549 Cancer Cells
AID  - 10.21873/anticanres.14245
DP  - 2020 May 01
TA  - Anticancer Research
PG  - 2739--2749
VI  - 40
IP  - 5
4099  - http://ar.iiarjournals.org/content/40/5/2739.short
4100  - http://ar.iiarjournals.org/content/40/5/2739.full
SO  - Anticancer Res2020 May 01; 40
AB  - Background/Aim: Ciprofloxacin has been used as an antibiotic in the clinic for decades. Recently, ciprofloxacin and its derivatives have shown promising anti-proliferative and cytotoxic activities against several malignant cells. The aim of this study was to investigate the effect of a new derivative of ciprofloxacin on colorectal cancer (HCT116) and non-small lung carcinoma (A549) cells. Materials and Methods: Cell viability was detected by the MTT assay. Flow cytometry was used to examine the cell cycle and apoptosis. Expression of bax, bcl2, p53 and p21 was investigated by qRT-PCR and western blotting. Results: Ciprofloxacin-derivative had an anti-proliferative effect on both cell lines in a concentration-dependent manner and caused cell cycle arrest at the G2/M phase and apoptosis. p53 and Bax proteins were overexpressed, while p21 and bcl2 gene expression was decreased after treatment with the ciprofloxacin derivative. Conclusion: This new ciprofloxacin derivative can be potentially used for the treatment of colorectal cancer and non-small lung carcinoma.