TY - JOUR T1 - Genetic Variations of <em>VEGFA</em> Gene Are Associated With Infiltration of Adjacent Tissues and the Clinical Outcome of Patients With Nasopharyngeal Carcinoma JF - Anticancer Research JO - Anticancer Res SP - 677 LP - 688 DO - 10.21873/anticanres.13997 VL - 40 IS - 2 AU - ELIZABETH PSOMA AU - GEORGIA-ANGELIKI KOLIOU AU - FOTEINOS-IOANNIS DIMITRAKOPOULOS AU - KYRIAKI PAPADOPOULOU AU - DIMITRA RONTOGIANNI AU - MATTHEOS BOBOS AU - ANASTASIOS VISVIKIS AU - PARIS A. KOSMIDIS AU - GEORGE FOUNTZILAS AU - JANNIS CONSTANTINIDIS AU - ANNA KALOGERA-FOUNTZILA Y1 - 2020/02/01 UR - http://ar.iiarjournals.org/content/40/2/677.abstract N2 - Background/Aim: The aim of the present study was to investigate the clinical significance of 7 single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor A (VEGFA), endothelin receptor type A (EDNRA), nibrin (NBS1) and Fas cell surface death receptor (FAS) genes in patients with nasopharyngeal carcinoma (NPC). Patients and Methods: Genomic DNA was extracted from the peripheral blood specimens of 60 patients. Genotyping of 4 VEGFA polymorphisms, namely VEGFA -1154 G/A (rs1570360), -2578 A/C (rs699947), -1498 C/T (rs833061) and +936 C/T (rs3025039), as well as EDNRA SNP p.His323 (rs5333), NBS1 p.E185Q (rs1805794) and FAS -671 A/G (rs1800682) was performed by using Sanger sequencing. Results: The VEGFA +936 CC genotype was more frequent in tumors with bilateral infiltration of pterygoid plates compared to those with ipsilateral (76.9% vs. 69.6%, p=0.008) and no infiltration of pterygoid plates (76.9% vs. 68.8%, p=0.023). VEGFA -2578, VEGFA -1154 and VEGFA +936 were significantly associated with infiltration to the pterygoid processes (p=0.011, p=0.041 and p=0.032, respectively). EDNRA H323H TT genotype was marginally associated with infiltration to the ipsilateral medial pterygoid muscles (p=0.045). A trend towards longer overall survival was observed for VEGFA -2578 CC as compared to AC (HR=0.24, p=0.060). Conclusion: The studied VEGFA SNPs seem to be associated with the local extension of the NPC and maybe with the clinical outcome of this patient group. ER -