TY - JOUR T1 - Approbation of the Cancer Treatment Approach Based on the Eradication of TAMRA+ Cancer Stem Cells in a Model of Murine Cyclophosphamide Resistant Lymphosarcoma JF - Anticancer Research JO - Anticancer Res SP - 795 LP - 805 DO - 10.21873/anticanres.14011 VL - 40 IS - 2 AU - POLINA E. KISARETOVA AU - SVETLANA S. KIRIKOVICH AU - GENRICH S. RITTER AU - YAROSLAV R. EFREMOV AU - OLEG S. TARANOV AU - TATYANA D. DUBATOLOVA AU - ANASTASIA S. PROSKURINA AU - EKATERINA A. POTTER AU - EVGENIA V. DOLGOVA AU - SERGEY V. SIDOROV AU - ALEKSANDR A. OSTANIN AU - ELENA R. CHERNYCH AU - SERGEY S. BOGACHEV Y1 - 2020/02/01 UR - http://ar.iiarjournals.org/content/40/2/795.abstract N2 - Background/Aim: We previously have described the “3+1” tumors cure approach consisting of individual time schedule of cyclophosphamide and dsDNA preparation administrations. The aim of the study was to adapt the “3+1” approach based on eradication of cancer stem cells to the model of murine ascitic cyclophosphamide-resistant lymphosarcoma (RLS). Materials and Methods: Adaptation of the “3+1” approach includes the identification of the timing to disrupt the tumorigenic potential of a certain tumor. Results: The proposed therapeutic scheme allowed complete reduction of primary RLS ascites in experimental animals. However, reduction of primary ascites due to the complementary action of cyclophosphamide and dsDNA was inevitably followed by the development of a secondary one, most likely arising from a solid carcinomatous formation in the peritoneal wall. Conclusion: The “3+1” approach resulted in the elimination of cancer stem cells, and, as a consequence, in the complete reduction of RLS ascites. ER -