TY - JOUR T1 - Evaluation of a Ultraviolet B Light Emitting Diode (LED) for Producing Vitamin D<sub>3</sub> in Human Skin JF - Anticancer Research JO - Anticancer Res SP - 719 LP - 722 DO - 10.21873/anticanres.14002 VL - 40 IS - 2 AU - ANGELINE J. VERONIKIS AU - MUHAMMET B. CEVIK AU - RACHEL H. ALLEN AU - ARASH SHIRVANI AU - AUDREY SUN AU - KELLY S. PERSONS AU - MICHAEL F. HOLICK Y1 - 2020/02/01 UR - http://ar.iiarjournals.org/content/40/2/719.abstract N2 - Aim: A commercially available light emitting diode (LED) that transmitted narrow band ultraviolet B (UVB) radiation was evaluated for its efficacy and efficiency to produce vitamin D3 in human skin. Materials and Methods: Human skin samples were obtained from surgical procedures. The LED had peak emission wavelength of 295 nm. Skin samples were exposed to the UVB-LED for varying times and then were analyzed by high-pressure liquid chromatography (HPLC) to determine the vitamin D3 content. Results: There was a statistically significant time- and dose-dependent increase in the percent of 7-dehydrocholesterol that was converted to vitamin D3 in the skin type II samples; 1.3%±0.5, 2.3%±0.6 and 4.5%±1.67 after exposure to 0.75 (11.7 mJ/cm2), 1.5 (23.4 mJ/cm2) and 3 (46.8 mJ/cm2) minimal erythemal doses (MEDs), respectively. Conclusion: The UVB-LED was effective and efficient in generating vitamin D3 in human skin, in vitro. The amount of vitamin D3 production increased in a dose-dependent fashion with increased UVB energy. UVB-LEDs can be developed for devices that can efficiently produce vitamin D3 in human skin. ER -