PT - JOURNAL ARTICLE AU - ANGELINE J. VERONIKIS AU - MUHAMMET B. CEVIK AU - RACHEL H. ALLEN AU - ARASH SHIRVANI AU - AUDREY SUN AU - KELLY S. PERSONS AU - MICHAEL F. HOLICK TI - Evaluation of a Ultraviolet B Light Emitting Diode (LED) for Producing Vitamin D<sub>3</sub> in Human Skin AID - 10.21873/anticanres.14002 DP - 2020 Feb 01 TA - Anticancer Research PG - 719--722 VI - 40 IP - 2 4099 - http://ar.iiarjournals.org/content/40/2/719.short 4100 - http://ar.iiarjournals.org/content/40/2/719.full SO - Anticancer Res2020 Feb 01; 40 AB - Aim: A commercially available light emitting diode (LED) that transmitted narrow band ultraviolet B (UVB) radiation was evaluated for its efficacy and efficiency to produce vitamin D3 in human skin. Materials and Methods: Human skin samples were obtained from surgical procedures. The LED had peak emission wavelength of 295 nm. Skin samples were exposed to the UVB-LED for varying times and then were analyzed by high-pressure liquid chromatography (HPLC) to determine the vitamin D3 content. Results: There was a statistically significant time- and dose-dependent increase in the percent of 7-dehydrocholesterol that was converted to vitamin D3 in the skin type II samples; 1.3%±0.5, 2.3%±0.6 and 4.5%±1.67 after exposure to 0.75 (11.7 mJ/cm2), 1.5 (23.4 mJ/cm2) and 3 (46.8 mJ/cm2) minimal erythemal doses (MEDs), respectively. Conclusion: The UVB-LED was effective and efficient in generating vitamin D3 in human skin, in vitro. The amount of vitamin D3 production increased in a dose-dependent fashion with increased UVB energy. UVB-LEDs can be developed for devices that can efficiently produce vitamin D3 in human skin.