@article {MOENG723, author = {SOKVISETH MOENG and SEUNG WAN SON and HYUN AH SEO and JONG SUN LEE and CHUNG KWON KIM and HYO JEONG KUH and JONG KOOK PARK}, title = {Luteolin-regulated MicroRNA-301-3p Targets Caspase-8 and Modulates TRAIL Sensitivity in PANC-1 Cells}, volume = {40}, number = {2}, pages = {723--731}, year = {2020}, doi = {10.21873/anticanres.14003}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: MicroRNAs (miRNAs) play regulatory roles in pancreatic ductal adenocarcinoma (PDAC). However, it is still required to identify the function of miRNA-301-3p in pancreatic cancer cells. Materials and Methods: Effects of luteolin on cell growth, TRAIL cytotoxicity, and miR-301-3p levels were evaluated. The role of miRNA-301-3p in regulating cell proliferation, target gene expression, and TRAIL cytotoxicity were studied. Results: The levels of miR-301-3p were down-regulated in PANC-1 cells exposed to luteolin, which inhibits the growth of PANC-1 cells and sensitizes cells to TRAIL. The knockdown of miR-301-3p attenuates cell proliferation and enhances TRAIL cytotoxicity. In addition, caspase-8 was directly targeted by miR-301-3p. Conclusion: Our findings unveil a critical biological function of miR-301-3p in regulating cell proliferation and elevating an antiproliferative effect of TRAIL on cancer cells. Our observation of miR-301-3p/caspase-8 relationship can also serve to clarify the role of miR-301-3p in other cancer types and related diseases.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/40/2/723}, eprint = {https://ar.iiarjournals.org/content/40/2/723.full.pdf}, journal = {Anticancer Research} }