RT Journal Article
SR Electronic
T1 Monitoring of Heat Shock Response and Phenotypic Changes in Hepatocellular Carcinoma After Heat Treatment
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 5393
OP 5401
DO 10.21873/anticanres.13733
VO 39
IS 10
A1 RYOSUKE ZAIMOKU
A1 TOMOHARU MIYASHITA
A1 HIDEHIRO TAJIMA
A1 HIROYUKI TAKAMURA
A1 AI HARASHIMA
A1 SEIICHI MUNESUE
A1 YASUHIKO YAMAMOTO
A1 ITASU NINOMIYA
A1 SACHIO FUSHIDA
A1 KENICHI HARADA
YR 2019
UL http://ar.iiarjournals.org/content/39/10/5393.abstract
AB Background/Aim: Local recurrence of hepatocellular carcinoma (HCC) after thermal coagulation therapy may be associated with an aggressive phenotypic change. This study focused on the thermal effects on HCC cells and evaluated the heat shock response and phenotypic changes after heat treatment. Materials and Methods: HepG2 and HuH7 cells were used. After heat treatment at 37-50°C for 5-30 min, we assessed their survival rate, induction of heat shock protein (HSP)70 promoter, proliferation rate, induction of the epithelial–mesenchymal transition (EMT) and cancer stem cell (CSC)-related markers. Results: Induction of HSP70 promoter per surviving cell was maximized after 10 min of heat treatment at 48°C. Induction of EMT and CSC-related markers was also observed. Conclusion: Sub-lethal heat treatment causes large heat shock response to surviving HCC cells and induce EMT-like and CSC-like phenotypic changes that might contribute to increased aggressiveness.