RT Journal Article SR Electronic T1 Linking Infection and Prostate Cancer Progression: Toll-like Receptor3 Stimulation Rewires Glucose Metabolism in Prostate Cells JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 5541 OP 5549 DO 10.21873/anticanres.13747 VO 39 IS 10 A1 MARIA CHIARA MAGNIFICO A1 ALBERTO MACONE A1 MARINA MARANI A1 AMANI BOUZIDI A1 GIORGIO GIARDINA A1 SERENA RINALDO A1 FRANCESCA CUTRUZZOLÀ A1 ALESSIO PAONE YR 2019 UL http://ar.iiarjournals.org/content/39/10/5541.abstract AB Background/Aim: The connection between prostate cancer and inflammation has been proposed many years ago, but very little is known about the metabolic adaptations of prostate cells in case of infection or inflammation. The aim of this study was to examine the effect of the stimulation of Toll-like receptor 3 (TLR3) on the metabolism of prostate cancer (PCa) cell lines and benign prostate cells. Materials and Methods: Cytofluorimetry, qRT-PCR, western blot and Gas-chromatography/Mass-spectrometry were used. Results: Reprogramming of glucose utilization involving hypoxia-inducible factor 1-alpha (HIF-1α) and the extracellular adenosine axis was observed. TLR3 stimulation synergized with adenosine receptor A2b on PCa cells, and induced a strong production of lactate, exacerbating the Warburg effect. Moreover, stimulation of benign prostate cells with poly I:C reduced lactate secretion, a characteristic typical of the neoplastic transformation. Conclusion: TLR3 stimulation promotes metabolic adaptations likely involved in the mechanisms of disease onset and progression.