PT - JOURNAL ARTICLE AU - TADASHI OHARA AU - TSUTOMU MORI TI - Antiproliferative Effects of Short-chain Fatty Acids on Human Colorectal Cancer Cells <em>via</em> Gene Expression Inhibition AID - 10.21873/anticanres.13647 DP - 2019 Sep 01 TA - Anticancer Research PG - 4659--4666 VI - 39 IP - 9 4099 - http://ar.iiarjournals.org/content/39/9/4659.short 4100 - http://ar.iiarjournals.org/content/39/9/4659.full SO - Anticancer Res2019 Sep 01; 39 AB - Background/Aim: Short-chain fatty acids (SCFAs) inhibit human colorectal cancer cell growth and tumorigenicity. We investigated the mechanism of the anti-proliferative effects of SCFAs on human colorectal cancer cells by examining their effects on gene expression. Materials and Methods: The DLD-1 cell line was cultured with different SCFAs. Gene groups whose expression levels decreased to &lt;50% or increased &gt;50% compared to untreated cells and the signalling pathways responsible for DLD-1 cell growth inhibition were identified and analyzed. Results: Genes whose expression levels decreased to ≤50% (791 genes) showed remarkable changes in gene function compared to genes whose expression levels increased ≥50%. These genes encode proteins involved in DNA replication and cell cycle/proliferation that contribute to major pathways responsible for suppression of colorectal carcinogenesis pathways. Conclusion: SCFAs inhibited the expression of genes encoding proteins involved in DNA replication and cell cycle/proliferation of human colorectal cancer cells and exerted antiproliferative activity via different pathways.