PT  - JOURNAL ARTICLE
AU  - CARLOS A. RUBIO
AU  - PETER T. SCHMIDT
TI  - The Normal Epithelium of Crypts Accruing Below Nonpolypoid Adenomas Thrives With Relocated Proliferating Cell-domains and p53-Up-regulated Cells
AID  - 10.21873/anticanres.13685
DP  - 2019 Sep 01
TA  - Anticancer Research
PG  - 4965--4970
VI  - 39
IP  - 9
4099  - http://ar.iiarjournals.org/content/39/9/4965.short
4100  - http://ar.iiarjournals.org/content/39/9/4965.full
SO  - Anticancer Res2019 Sep 01; 39
AB  - Background/Aim: Colonic crypts with normal epithelium albeit with corrupted shapes (CCS) were previously found beneath nonpolypoid adenomas (NPA). This study aimed to analyze the distribution of proliferating cells (PC) and p53-up-regulated cells in CCS. Materials and Methods: Sections from 48 NPA were immunostained with the proliferating-marker Ki67 and against the tumor-suppressor protein p53. Results: Asymmetric-haphazardly distributed PC were found in 87.5% of the NPA, continuous PC-domains in 8.3%, asymmetric-haphazardly distributed single PC in 4.2% and p53-up-regulated cells in 29.2%. In 12 controls, the normal-shaped crypts revealed symmetrically-distributed PC-domains in their lower thirds, and no p53-up-regulated cells. Conclusion: The normal epithelium that lines the CCS below NPA, thrives with relocated PC-domains, and with occasional p53-up-regulated cells. These findings strongly suggest that the normal epithelium of CCS beneath NPA might harbor somatic mutations. The accretion of putative mutated CCS might play an important role in the evolution of nonpolypoid adenomas in the human colon.