TY - JOUR T1 - Ro 90-7501 Is a Novel Radiosensitizer for Cervical Cancer Cells that Inhibits ATM Phosphorylation JF - Anticancer Research JO - Anticancer Res SP - 4805 LP - 4810 DO - 10.21873/anticanres.13665 VL - 39 IS - 9 AU - KEISUKE TAMARI AU - KEISUKE SANO AU - ZHIHAO LI AU - YUJI SEO AU - KEISUKE OTANI AU - SHOTARO TATEKAWA AU - MASAYASU TORATANI AU - YUJI TAKAOKA AU - YUTAKA TAKAHASHI AU - KAZUMASA MINAMI AU - FUMIAKI ISOHASHI AU - MASAHIKO KOIZUMI AU - KAZUHIKO OGAWA Y1 - 2019/09/01 UR - http://ar.iiarjournals.org/content/39/9/4805.abstract N2 - Background/Aim: Ro 90-7501 has been reported as an inhibitor of the amyloid β42 fibril assembly that is associated with Alzheimer's disease. The present study aimed to elucidate the radiosensitizing effects of Ro 90-7501 and focused on ATM signaling after irradiation. Materials and Methods: Clonogenic survival, apoptosis, and cell-cycle assays as well as western blotting were performed in HeLa cells treated with irradiation and Ro 90-7501. Tumor growth delay assay was also performed using BALB/c-nu mice. Results: The combination of irradiation with Ro 90-7501 showed significant radiosensitizing effects in clonogenic survival and tumor growth delay assays. Ro 90-7501 significantly increased apoptosis and impaired cell cycle after irradiation. Western blotting showed that Ro 90-7501 suppressed the phosphorylation of ATM and its downstream proteins, such as H2AX, Chk1, and Chk2, after irradiation. Conclusion: Ro 90-7501 inhibits DNA damage response by inhibiting ATM and has significant radiosensitizing effects on cervical cancer cells. ER -