PT - JOURNAL ARTICLE AU - TASUKU KIYUNA AU - YASUNORI TOME AU - KENTARO MIYAKE AU - TAKASHI MURAKAMI AU - HIROMICHI OSHIRO AU - KENTARO IGARASHI AU - KEI KAWAGUCHI AU - JOHN HSU AU - MANISH SINGH AU - YUNFENG LI AU - SCOTT NELSON AU - MICHAEL BOUVET AU - SHREE RAM SINGH AU - FUMINORI KANAYA AU - ROBERT M. HOFFMAN TI - Eribulin Suppressed Cisplatinum- and Doxorubicin-resistant Recurrent Lung Metastatic Osteosarcoma in a Patient-derived Orthotopic Xenograft Mouse Model AID - 10.21873/anticanres.13661 DP - 2019 Sep 01 TA - Anticancer Research PG - 4775--4779 VI - 39 IP - 9 4099 - http://ar.iiarjournals.org/content/39/9/4775.short 4100 - http://ar.iiarjournals.org/content/39/9/4775.full SO - Anticancer Res2019 Sep 01; 39 AB - Background: Osteosarcoma is a recalcitrant disease treated with surgery and intensive chemotherapy as standard. The 5-year survival rate of patients with relapsed and lung metastatic osteosarcoma is as low as 20%. Materials and Methods: A 16-year-old patient developed left distal femoral high-grade osteosarcoma and underwent cisplatinum-based neoadjuvant chemotherapy and surgery. From the resected tumor, a patient-derived orthotopic xenograft (PDOX) model was established in the femur of nude mice. PDOX models were randomized into the following groups: untreated control, or treatment with doxorubicin (3 mg/kg, i.p., weekly for 14 days), sunitinib (40 mg/kg, oral gavage, daily for 14 days), pazopanib (100 mg/kg, oral gavage, daily for 14 days), temozolomide(25 mg/kg, oral gavage, daily for 14 days), and eribulin (1.5 mg/kg, i.p., daily for 14 days). Tumor volume and body weight were monitored twice a week. Results: The osteosarcoma PDOX was resistant to doxorubicin, sunitinib, and pazopanib. In contrast, eribulin and temozolomide arrested tumor growth. Conclusion: This study demonstrated the utility of the PDOX model in allowing effective from non-effective drugs to be distinguished in a model in which the tumor was growing on the organ corresponding to that of the patient.