@article {TANAKA6575, author = {HIDEKAZU TANAKA and MASAYA KAWAGUCHI and SHINICHI SHODA and TOSHIHARU MIYOSHI and RYOTA IWASAKI and FUMINORI HYODO and TAKASHI MORI and AKIRA HARA and HIROYUKI TOMITA and MASAYUKI MATSUO}, title = {Nuclear Accumulation of β-Catenin in Cancer Stem Cell Radioresistance and Stemness in Human Colon Cancer}, volume = {39}, number = {12}, pages = {6575--6583}, year = {2019}, doi = {10.21873/anticanres.13873}, publisher = {International Institute of Anticancer Research}, abstract = {Background/Aim: The aim of this study was to examine whether the Wnt/β-catenin signal activation is a cause of radioresistance in colon cancer by assessing the β-catenin localization and its correlation with cancer stem cells (CSCs). Materials and Methods: The nuclear levels of β-catenin, the hallmark of Wnt activation, were analyzed in HCT116 and SW480 cells by immunohistochemistry, before and after irradiation. Further, we assessed CSC populations by staining for aldehyde dehydrogenase-1 (ALDH1) and CD44. Results: β-catenin was localized predominantly in the nucleus and plasma membrane in SW480 and HCT116 cells, respectively. Compared to HCT116 cells, SW480 cells displayed higher Wnt activation. At 24 h after irradiation, most of the DSBs in SW480 cells were repaired, but were still present in HCT116 cells. Additionally, compared to HCT116 cells, a significantly higher proportion of SW480 cells were ALDH1- and CD44-positive. Conclusion: Colon cancers with nuclear β-catenin accumulation demonstrated greater radio-resistance with a higher number of CSCs.}, issn = {0250-7005}, URL = {https://ar.iiarjournals.org/content/39/12/6575}, eprint = {https://ar.iiarjournals.org/content/39/12/6575.full.pdf}, journal = {Anticancer Research} }