RT Journal Article
SR Electronic
T1 Bis(4-hydroxy-2H-chromen-2-one) Coumarin Induces Apoptosis in MCF-7 Human Breast Cancer Cells Through Aromatase Inhibition
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6107
OP 6114
DO 10.21873/anticanres.13818
VO 39
IS 11
A1 RAMDANI, LAMIA HAMDAN
A1 TALHI, OUALID
A1 DECOMBAT, CAROLINE
A1 VERMERIE, MARION
A1 BERRY, ALEXANDRE
A1 SILVA, ARTUR
A1 BACHARI, KHALDOUN
A1 VASSON, MARIE-PAULE
A1 DELORT, LAETITIA
A1 CALDEFIE-CHÉZET, FLORENCE
YR 2019
UL http://ar.iiarjournals.org/content/39/11/6107.abstract
AB Aim: The present investigation aimed to examine the therapeutic potential of the new coumarin derivative bis(4-hydroxy-2H-chromen-2-one) coumarin (4HC) against breast cancer. Materials and Methods: For this purpose, the effects of 4HC treatment on the proliferation of MCF-7 breast cancer cells and on MCF-10a non-cancerous cells were evaluated using a fluorescent assay. Cell cycle distribution and apoptosis were measured by image cytometry. The expression level of aromatase (CYP19A1) and apoptosis-related genes were determined by real-time PCR. Results: MCF-7 mammary cancer cell proliferation was significantly decreased within 24 h after treatment with 4HC at 50 μM, while no effect was observed on the viability of MCF-10a non-cancerous mammary cells. 4HC also increased the percentage of the cells in the G2/M phase, inducing apoptosis. Real-time PCR revealed that 4HC induced MCF-7 mortality through an up-regulation of Bax and a down-regulation of Bcl-2, resulting in an increase in caspase-3 gene expression. The increased expression of apoptosis-related genes was accompanied by a decrease in CYP19A1 gene expression. Conclusion: 4HC selectively inhibits proliferation of MCF-7cells in vitro. Moreover, 4HC has inhibitory effects on aromatase gene expression and promoting effects on apoptosis, in MCF-7 cells.