<?xml version='1.0' encoding='UTF-8'?><xml><records><record><source-app name="HighWire" version="7.x">Drupal-HighWire</source-app><ref-type name="Journal Article">17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">RAMDANI, LAMIA HAMDAN</style></author><author><style face="normal" font="default" size="100%">TALHI, OUALID</style></author><author><style face="normal" font="default" size="100%">DECOMBAT, CAROLINE</style></author><author><style face="normal" font="default" size="100%">VERMERIE, MARION</style></author><author><style face="normal" font="default" size="100%">BERRY, ALEXANDRE</style></author><author><style face="normal" font="default" size="100%">SILVA, ARTUR</style></author><author><style face="normal" font="default" size="100%">BACHARI, KHALDOUN</style></author><author><style face="normal" font="default" size="100%">VASSON, MARIE-PAULE</style></author><author><style face="normal" font="default" size="100%">DELORT, LAETITIA</style></author><author><style face="normal" font="default" size="100%">CALDEFIE-CHÉZET, FLORENCE</style></author></authors><secondary-authors></secondary-authors></contributors><titles><title><style face="normal" font="default" size="100%">Bis(4-hydroxy-2H-chromen-2-one) Coumarin Induces Apoptosis in MCF-7 Human Breast Cancer Cells Through Aromatase Inhibition</style></title><secondary-title><style face="normal" font="default" size="100%">Anticancer Research</style></secondary-title></titles><dates><year><style  face="normal" font="default" size="100%">2019</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2019-11-01 00:00:00</style></date></pub-dates></dates><pages><style  face="normal" font="default" size="100%">6107-6114</style></pages><doi><style  face="normal" font="default" size="100%">10.21873/anticanres.13818</style></doi><volume><style face="normal" font="default" size="100%">39</style></volume><issue><style face="normal" font="default" size="100%">11</style></issue><abstract><style  face="normal" font="default" size="100%">Aim: The present investigation aimed to examine the therapeutic potential of the new coumarin derivative bis(4-hydroxy-2H-chromen-2-one) coumarin (4HC) against breast cancer. Materials and Methods: For this purpose, the effects of 4HC treatment on the proliferation of MCF-7 breast cancer cells and on MCF-10a non-cancerous cells were evaluated using a fluorescent assay. Cell cycle distribution and apoptosis were measured by image cytometry. The expression level of aromatase (CYP19A1) and apoptosis-related genes were determined by real-time PCR. Results: MCF-7 mammary cancer cell proliferation was significantly decreased within 24 h after treatment with 4HC at 50 μM, while no effect was observed on the viability of MCF-10a non-cancerous mammary cells. 4HC also increased the percentage of the cells in the G2/M phase, inducing apoptosis. Real-time PCR revealed that 4HC induced MCF-7 mortality through an up-regulation of Bax and a down-regulation of Bcl-2, resulting in an increase in caspase-3 gene expression. The increased expression of apoptosis-related genes was accompanied by a decrease in CYP19A1 gene expression. Conclusion: 4HC selectively inhibits proliferation of MCF-7cells in vitro. Moreover, 4HC has inhibitory effects on aromatase gene expression and promoting effects on apoptosis, in MCF-7 cells.</style></abstract></record></records></xml>