RT Journal Article SR Electronic T1 Expression, Function, and Prognostic Value of MAGE-D4 Protein in Esophageal Squamous Cell Carcinoma JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6015 OP 6023 DO 10.21873/anticanres.13807 VO 39 IS 11 A1 YASUO UNO A1 MITSURO KANDA A1 YUSUKE SATO A1 DAI SHIMIZU A1 SHINICHI UMEDA A1 NORIFUMI HATTORI A1 MASAMICHI HAYASHI A1 CHIE TANAKA A1 DAISUKE KOBAYASHI A1 SUGURU YAMADA A1 GORO NAKAYAMA A1 SATORU MOTOYAMA A1 MASAHIKO KOIKE A1 YASUHIRO KODERA YR 2019 UL http://ar.iiarjournals.org/content/39/11/6015.abstract AB Background/Aim: We previously reported that expression of melanoma-associated antigen (MAGE)-D4 mRNA was a prognostic factor for esophageal squamous cell carcinoma (ESCC). The aim of this study was to validate the expression of MAGE-D4 in two additional patient cohorts, and to investigate its biological functions. Materials and Methods: The role of MAGE-D4 in cell proliferation, adhesion, and migration was determined by gene knockdown experiments in the KYSE590 cell line. MAGE-D4 protein expression was analyzed in ESCC tissues by immunohistochemistry. A second validation cohort consisted of an ESCC mRNA dataset from The Cancer Genome Atlas. Results: Knockdown of MAGE-D4 significantly decreased cell proliferation and migration. Expression of MAGE-D4 protein was significantly associated with disease-free survival. In the second validation cohort, high MAGE-D4 mRNA expression was associated with significantly shorter overall survival and disease-free survival. Conclusion: MAGE-D4 plays an important role in the malignant behavior of ESCC. MAGE-D4 was validated as a prognostic indicator in two independent ESCC patient cohorts.