TY - JOUR T1 - Expression, Function, and Prognostic Value of MAGE-D4 Protein in Esophageal Squamous Cell Carcinoma JF - Anticancer Research JO - Anticancer Res SP - 6015 LP - 6023 DO - 10.21873/anticanres.13807 VL - 39 IS - 11 AU - YASUO UNO AU - MITSURO KANDA AU - YUSUKE SATO AU - DAI SHIMIZU AU - SHINICHI UMEDA AU - NORIFUMI HATTORI AU - MASAMICHI HAYASHI AU - CHIE TANAKA AU - DAISUKE KOBAYASHI AU - SUGURU YAMADA AU - GORO NAKAYAMA AU - SATORU MOTOYAMA AU - MASAHIKO KOIKE AU - YASUHIRO KODERA Y1 - 2019/11/01 UR - http://ar.iiarjournals.org/content/39/11/6015.abstract N2 - Background/Aim: We previously reported that expression of melanoma-associated antigen (MAGE)-D4 mRNA was a prognostic factor for esophageal squamous cell carcinoma (ESCC). The aim of this study was to validate the expression of MAGE-D4 in two additional patient cohorts, and to investigate its biological functions. Materials and Methods: The role of MAGE-D4 in cell proliferation, adhesion, and migration was determined by gene knockdown experiments in the KYSE590 cell line. MAGE-D4 protein expression was analyzed in ESCC tissues by immunohistochemistry. A second validation cohort consisted of an ESCC mRNA dataset from The Cancer Genome Atlas. Results: Knockdown of MAGE-D4 significantly decreased cell proliferation and migration. Expression of MAGE-D4 protein was significantly associated with disease-free survival. In the second validation cohort, high MAGE-D4 mRNA expression was associated with significantly shorter overall survival and disease-free survival. Conclusion: MAGE-D4 plays an important role in the malignant behavior of ESCC. MAGE-D4 was validated as a prognostic indicator in two independent ESCC patient cohorts. ER -