RT Journal Article SR Electronic T1 Expression of Serpin B9 as a Prognostic Factor of Colorectal Cancer JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 6063 OP 6066 DO 10.21873/anticanres.13813 VO 39 IS 11 A1 ONDREJ VYCITAL A1 PAVEL PITULE A1 PETR HOSEK A1 TOMAS KRIZ A1 VLADISLAV TRESKA A1 VACLAV LISKA YR 2019 UL http://ar.iiarjournals.org/content/39/11/6063.abstract AB Background/Aim: Treatment of colorectal cancer (CRC) does not reflect immune interactions between tumours and macro-organisms. Serpin B9 is known as an inhibitor of Granzyme B. The aim of this study was to evaluate the impact of the expression of Serpin B9 in CRC and healthy colon tissue on prognosis. Patients and Methods: This retrospective study included 74 CRC patients in all stages. Analysis of gene expression was performed with quantitative polymerase chain reaction with reverse transcription using specific primers and master mix Xceed qPCR SG. Expression was normalized to the reference genes GAPDH, ACTB, and PSMC. Results: Increased expression of Serpin B9 in healthy tissue was significantly associated with longer overall survival (OS). This association was found both in all patients and in the group of patients with distant metastases. Conclusion: The presented results support previous evidence of positive influence of the interaction between immune system and tumour on the prognosis of CRC.