TY - JOUR T1 - Expression of Kita-Kyushu Lung Cancer Antigen-1 as Detected by a Novel Monoclonal Antibody in Gastric Cancer JF - Anticancer Research JO - Anticancer Res SP - 6259 LP - 6263 DO - 10.21873/anticanres.13835 VL - 39 IS - 11 AU - YOSHIHITO TAKAHASHI AU - TAKASHI FUKUYAMA AU - NOBUE FUTAWATARI AU - YOSHINOBU ICHIKI AU - HARUKI OHMIYA AU - MOTOHIRO CHUMAN AU - TETSURO YAMASHITA AU - NAOKI HIKI AU - YUSUKE KUMAMOTO AU - HITOSHI YAMAZAKI AU - HIROSHI NONOGUCHI AU - YATSUSHI NISHI AU - NORITADA KOBAYASHI Y1 - 2019/11/01 UR - http://ar.iiarjournals.org/content/39/11/6259.abstract N2 - Background/Aim: Kita-Kyushu lung cancer antigen-1 (KK-LC-1) is a known cancer/testis antigen. Our group has previously shown KK-LC-1 gene expression in gastric cancer. However, could not be detected the KK-LC-1 protein due to the lack of an appropriate antibody. Here, we assessed our original monoclonal antibody (Kmab34B3) and, using it, assessed the expression of KK-LC-1 in gastric cancer. Patients and Methods: We evaluated an original monoclonal antibody against KK-LC-1 (Kmab34B3), and used this antibody to compare KK-LC-1 protein expression in tumour and non-tumour stomach cells from gastric cancer patients. Results: Kmab34B3 stained testicular germ cells, and tumour cells in nine out of 11 (82%) specimens. In non-tumorous areas, Kmab34B3 stained 13 out of 29 (45%) pyloric gland specimens. Furthermore, Kmab34B3 also stained intestinal metaplasia positive and negative areas. Conclusion: Kmab34B3 was able to detect KK-LC-1 protein within tumour cells and the pyloric gland where the gene has been shown to be expressed. Therefore, it might be an attractive tool for detecting KK-LC-1 expression in precancerous and cancerous stomach cells. ER -