TY - JOUR T1 - G3BP1 Depletion Increases Radiosensitisation by Inducing Oxidative Stress in Response to DNA Damage JF - Anticancer Research JO - Anticancer Res SP - 6087 LP - 6095 DO - 10.21873/anticanres.13816 VL - 39 IS - 11 AU - EUGENE CHO AU - THOA THI THAN AU - SU-HYEON KIM AU - EUN-RAN PARK AU - MI-YEON KIM AU - KEE HO LEE AU - HYUN JIN SHIN Y1 - 2019/11/01 UR - http://ar.iiarjournals.org/content/39/11/6087.abstract N2 - Background: RAS GTPase-activating protein-binding protein (G3BP1) is an RNA-binding protein that is essential for assembling stress granules. Many functions related to the survival and progression of cancer have been reported. The current study aimed to investigate the role of G3BP1 in radio-sensitisation of cancer cells. Materials and Methods: Radiation sensitivity and chemosensitivity were analysed in A549 and H460 cells transfected with G3BP1 siRNAs, and N-acetyl-L-cysteine (NAC) was used to elucidate the involvement of reactive oxygen species (ROS). Results: G3BP1 depletion sensitised lung cancer cell lines to radiation, and the effect was related to ROS. G3BP1 depletion impaired the intracellular ROS scavenging system and NAC abolished the radiation-sensitive phenotypes caused by G3BP1 depletion. Conclusion: The study suggested G3BP1 as a promising target for radio- and chemosensitisation of lung cancer. ER -