TY - JOUR T1 - DJ-1 Contributes to Self-renewal of Stem Cells in the U87-MG Glioblastoma Cell Line JF - Anticancer Research JO - Anticancer Res SP - 5983 LP - 5990 DO - 10.21873/anticanres.13803 VL - 39 IS - 11 AU - YUKI TODA AU - RYOSUKE YOSHIMURA AU - MASAO ITAHARA AU - YURI IMAI AU - KANAE YAMADA AU - TOMOKO UNO AU - SUSUMU NAKATA AU - SHIGEKUNI HOSOGI AU - KAZUYUKI TAKATA AU - EISHI ASHIHARA Y1 - 2019/11/01 UR - http://ar.iiarjournals.org/content/39/11/5983.abstract N2 - Background/Aim: DJ-1, an oncogenic molecule, helps to maintain somatic stem cells by reducing the intracellular level of reactive oxygen species (ROS). This study investigated the role of DJ-1 in glioma stem cells (GSCs). Materials and Methods: U87-MG (U87) and U251-MG (U251) glioblastoma cell lines that express wild-type and mutant p53, respectively, were used. These were cultured with DJ-1-targeting siRNA and subjected to a variety of in vitro experiments or intracranial transplantation into nude mice. Results: Knockdown of DJ-1 reduced clonogenicity only in U87 cells, which was rescued by p53 depletion. ROS accumulated in DJ-1-depleted cells, although treatment with N-acetyl cysteine, which quenches ROS, did not affect exhaustion of CSCs among U87 cells by DJ-1 knockdown. In a serial transplantation study, DJ-1 knockdown prolonged the survival of mice in secondary transplantation. Conclusion: DJ-1 plays a pivotal role in maintenance of stem cell self-renewal in the U87 cell line. ER -