PT  - JOURNAL ARTICLE
AU  - YUKI TODA
AU  - RYOSUKE YOSHIMURA
AU  - MASAO ITAHARA
AU  - YURI IMAI
AU  - KANAE YAMADA
AU  - TOMOKO UNO
AU  - SUSUMU NAKATA
AU  - SHIGEKUNI HOSOGI
AU  - KAZUYUKI TAKATA
AU  - EISHI ASHIHARA
TI  - DJ-1 Contributes to Self-renewal of Stem Cells in the U87-MG Glioblastoma Cell Line
AID  - 10.21873/anticanres.13803
DP  - 2019 Nov 01
TA  - Anticancer Research
PG  - 5983--5990
VI  - 39
IP  - 11
4099  - http://ar.iiarjournals.org/content/39/11/5983.short
4100  - http://ar.iiarjournals.org/content/39/11/5983.full
SO  - Anticancer Res2019 Nov 01; 39
AB  - Background/Aim: DJ-1, an oncogenic molecule, helps to maintain somatic stem cells by reducing the intracellular level of reactive oxygen species (ROS). This study investigated the role of DJ-1 in glioma stem cells (GSCs). Materials and Methods: U87-MG (U87) and U251-MG (U251) glioblastoma cell lines that express wild-type and mutant p53, respectively, were used. These were cultured with DJ-1-targeting siRNA and subjected to a variety of in vitro experiments or intracranial transplantation into nude mice. Results: Knockdown of DJ-1 reduced clonogenicity only in U87 cells, which was rescued by p53 depletion. ROS accumulated in DJ-1-depleted cells, although treatment with N-acetyl cysteine, which quenches ROS, did not affect exhaustion of CSCs among U87 cells by DJ-1 knockdown. In a serial transplantation study, DJ-1 knockdown prolonged the survival of mice in secondary transplantation. Conclusion: DJ-1 plays a pivotal role in maintenance of stem cell self-renewal in the U87 cell line.