RT Journal Article
SR Electronic
T1 Plasma Levels and Tissue Expression of Selected Cytokines, Metalloproteinases and Tissue Inhibitors in Patients With Cervical Cancer
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 6403
OP 6412
DO 10.21873/anticanres.13854
VO 39
IS 11
A1 IWONA SIDORKIEWICZ
A1 BARBARA PISKÓR
A1 EMILIA DĄBROWSKA
A1 KATARZYNA GUZIŃSKA-USTYMOWICZ
A1 ANNA PRYCZYNICZ
A1 MONIKA ZBUCKA-KRĘTOWSKA
A1 SŁAWOMIR ŁAWICKI
YR 2019
UL http://ar.iiarjournals.org/content/39/11/6403.abstract
AB Background: Cytokines, metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) take part in many processes involved in tumor progression and invasion such as degradation of the extracellular matrix, influence on immune cells associated with tumor tissue, and angiogenesis. Thus, the aim of this study was to compare the concentration of plasma levels and tissue expression of macrophage colony-stimulating factor (M-CSF), vascular endothelial growth factor (VEGF), matrix metalloproteinases (MMP)-2 and MMP9, and their tissue inhibitors TIMP1 and TIMP2 in patients with cervical cancer, patients with high-grade cervical intraepithelial dysplasia (CIN3) and patients with ectropion. Patients and Methods: Concentration and expression of all tested parameters was measured in serum with enzyme-linked immunosorbent assay (ELISA) and in tissue with immunohistochemistry method. Results: The epithelial expression of M-CSF and TIMP1 in cancer tissue was much stronger as compared to that in ectropion and CIN3. In the case of MMP2, lack of or weak expression in epithelial cells was observed in all tested groups. Our studies showed statistical differences of tested parameters in tissue expression and in plasma concentrations in patients with cervical cancer, patients with CIN3 and patients with ectropion. Moreover, data revealed positive correlation between plasma level and cervical cancer cell expression of VEGF. Conclusion: Our findings indicate a potential role of all the proteins tested here in cervical cancer diagnosis, especially VEGF. However, further studies will show whether they play a role in the progression of cancerous changes in epithelial tissue of the cervix.