TY - JOUR T1 - M-CSF Receptor Antagonists Inhibit the Initiation and Progression of Hepatocellular Carcinoma in Mice JF - Anticancer Research JO - Anticancer Res SP - 4787 LP - 4794 DO - 10.21873/anticanres.13663 VL - 39 IS - 9 AU - YOSHIHIRO AKAZAWA AU - HIROSHI KONO AU - MICHIO HARA AU - SHINJI FURUYA AU - YUUKI NAKATA AU - HIROYUKI WAKANA AU - HISATAKA FUKUSHIMA AU - CHAO SUN AU - HIDEKI FUJII AU - DAISUKE ICHIKAWA Y1 - 2019/09/01 UR - http://ar.iiarjournals.org/content/39/9/4787.abstract N2 - Background/Aim: The aim of this study was to investigate the effects of the macrophage colony-stimulating factor (M-CSF) receptor antagonist on hepatic carcinogenesis in mice. Materials and Methods: Mice were injected with diethylnitrosamine (DEN) and treated with M-CSF receptor antagonist GW2580 (GW) or a saline vehicle just after (early treated group) or 2 weeks after (late treated group) DEN injection. Animals were sacrificed after 28 weeks and incidence of tumor was assessed. Isolated Kupffer cells were co-cultured with M-CSF in the presence or absence of GW, and the concentration of VEGF was measured. Results: The incidence of tumors was significantly blunted both in the early- and the late-treated groups. In addition, angiogenesis within the tumor was also suppressed in both groups. The concentration of VEGF increased in Kupffer cells treated with M-CSF compared to those cultured without M-CSF. This increase was blunted by GW. Conclusion: M-CSF and its receptor could be novel molecular targets for hepatocellular carcinoma. ER -