PT  - JOURNAL ARTICLE
AU  - YESOL KIM
AU  - YEON-SUK KIM
AU  - MINJEONG KIM
AU  - JUN-MO KIM
AU  - HAE-HYEOG LEE
AU  - TAE-HEE KIM
TI  - Thioredoxin-interacting Protein (TXNIP) Mediates Thioredoxin-dependent Antioxidant Mechanism in Endometrial Cancer Cells Treated With 1α,25-dihydroxyvitamin D<sub>3</sub>
AID  - 10.21873/anticanres.13664
DP  - 2019 Sep 01
TA  - Anticancer Research
PG  - 4795--4803
VI  - 39
IP  - 9
4099  - http://ar.iiarjournals.org/content/39/9/4795.short
4100  - http://ar.iiarjournals.org/content/39/9/4795.full
SO  - Anticancer Res2019 Sep 01; 39
AB  - Background/Aim: To determine the mechanism of vitamin D3-induced modulation of antioxidant-related factors in endometrial cancer, we investigated their role in apoptosis of human endometrial cancer cells exposed to vitamin D3. Materials and Methods: The survival rate of human endometrial cancer cells was estimated after treatment with activated vitamin D3. Reactive oxygen species (ROS) levels were measured using flow cytometry. The levels of VDR, Trx, TXNIP and apoptosis-related proteins were investigated using western blotting and immunocytochemistry in human tissues. Results: Treatment with D3 induced apoptotic cell death and cell-cycle arrest by increasing ROS concentration. Vitamin D3 inhibited proliferation of human endometrial cancer cells. It regulated intracellular ROS concentration in endometrial cancer cells via increased TXNIP expression. Conclusion: Antioxidant regulation via TXNIP is an important cell death mechanism in human endometrial cancer, and occurs via induction by vitamin D3.