PT  - JOURNAL ARTICLE
AU  - TOMOYUKI SATAKE
AU  - ATSUSHI SUETSUGU
AU  - MIKI NAKAMURA
AU  - TAKAHIRO KUNISADA
AU  - SHIGETOYO SAJI
AU  - HISATAKA MORIWAKI
AU  - MASAHITO SHIMIZU
AU  - ROBERT M. HOFFMAN
TI  - Color-coded Imaging of the Fate of Cancer-cell-derived Exosomes During Pancreatic Cancer Metastases in a Nude-mouse Model
AID  - 10.21873/anticanres.13561
DP  - 2019 Aug 01
TA  - Anticancer Research
PG  - 4055--4060
VI  - 39
IP  - 8
4099  - http://ar.iiarjournals.org/content/39/8/4055.short
4100  - http://ar.iiarjournals.org/content/39/8/4055.full
SO  - Anticancer Res2019 Aug 01; 39
AB  - Background/Aim: Tumor-derived exosomes play important roles in tumor metastases. In this report, we observed the fate of tumor-derived exosomes in pancreatic cancer metastatic nude-mouse models using color-coded imaging. Materials and Methods: Mia-PaCa-2 human pancreatic cancer cells expressing red fluorescent protein (RFP) were transduced by exosome-specific pCT-CD63-green fluorescent protein (GFP) and injected in the spleen of nude mice. Results: Four weeks after injection of these cells into the spleen, liver metastases developed and tumor-derived exosomes were observed within the metastatic cancer cells and in Kupffer cells. Furthermore, tumor-derived exosomes diffused to bone marrow and lung cells, especially macrophages, without any metastases present. Conclusion: In the present study, we visualized the distribution of cancer-derived exosomes for the first time at the cellular level, in a pancreatic-cancer metastatic model.