RT Journal Article SR Electronic T1 Elimination of Ras Suppressor-1 from Hepatocellular Carcinoma Cells Hinders their In Vitro Metastatic Properties JF Anticancer Research JO Anticancer Res FD International Institute of Anticancer Research SP 1509 OP 1512 VO 35 IS 3 A1 GKRETSI, VASILIKI A1 BOGDANOS, DIMITRIOS P. YR 2015 UL http://ar.iiarjournals.org/content/35/3/1509.abstract AB Background/Aim: Extracellular matrix (ECM) is of great significance for homeostasis in the liver. In fact, one of the stages leading to hepatocellular carcinoma (HCC) includes accumulation of excess ECM. Ras Suppressor-1 (RSU-1) is localized in the cell-ECM adhesions but its role in HCC is unexplored. Materials and Methods: We investigated the expression and role of RSU-1 in two HCC cell lines that differ in aggressiveness; non-invasive Alexander cells and highly invasive HepG2 cells. Results: Our results showed that RSU-1 expression is elevated in HepG2 cells both at the mRNA and protein level, while its silencing leads to increased cell proliferation in both cell lines. Interestingly, RSU-1 depletion from highly invasive HepG2 cells reduces cell adhesion and invasion. Conclusion: This is the first study to provide in vitro evidence for the involvement of RSU-1 in HCC cell invasive behavior.