RT Journal Article
SR Electronic
T1 Induction of Cell Death by Combination Treatment with Cisplatin and 5-Fluorouracil in a Human Oral Squamous Cell Carcinoma Cell Line
JF Anticancer Research
JO Anticancer Res
FD International Institute of Anticancer Research
SP 3331
OP 3337
VO 27
IS 5A
A1 OKAMURA, MASAHIKO
A1 KOBAYASHI, MASAKI
A1 SUZUKI, FUMIKA
A1 SHIMADA, JUN
A1 SAKAGAMI, HIROSHI
YR 2007
UL http://ar.iiarjournals.org/content/27/5A/3331.abstract
AB The possible apoptosis-inducing activity of several sequential treatments of cisplatin (CDDP) and 5-fluorouracil (5-FU) against the human oral squamous cell carcinoma HSC-2 cell line was investigated. The following three combination treatments (CT) were used: simultaneous treatment with CDDP and 5-FU (for 72 hours) (CT-1), CDDP treatment (24 hours) followed by 5-FU treatment (48 hours) (CT-2) and 5-FU treatment (24 hours) followed by CDDP treatment (48 hours) (CT-3). CT-1 produced the highest cytotoxicity, followed by CT-3 and CT-2. No treatment induced any detectable internucleosomal DNA fragmentation, and caspase-3,-8 and -9 were activated to a much lesser extent than that attained using actinomycin D. High-performance liquid chromatography analysis demonstrated that 5-FU, as well as CT-1 and CT-2, preferentially reduced the intracellular concentration of putrescine. These results suggest that simultaneous treatment with CDDP and 5-FU induces lower level of apoptotic cell death in HSC-2 cells. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved