PT  - JOURNAL ARTICLE
AU  - OKAMURA, MASAHIKO
AU  - KOBAYASHI, MASAKI
AU  - SUZUKI, FUMIKA
AU  - SHIMADA, JUN
AU  - SAKAGAMI, HIROSHI
TI  - Induction of Cell Death by Combination Treatment with Cisplatin and 5-Fluorouracil in a Human Oral Squamous Cell Carcinoma Cell Line
DP  - 2007 Sep 01
TA  - Anticancer Research
PG  - 3331--3337
VI  - 27
IP  - 5A
4099  - http://ar.iiarjournals.org/content/27/5A/3331.short
4100  - http://ar.iiarjournals.org/content/27/5A/3331.full
SO  - Anticancer Res2007 Sep 01; 27
AB  - The possible apoptosis-inducing activity of several sequential treatments of cisplatin (CDDP) and 5-fluorouracil (5-FU) against the human oral squamous cell carcinoma HSC-2 cell line was investigated. The following three combination treatments (CT) were used: simultaneous treatment with CDDP and 5-FU (for 72 hours) (CT-1), CDDP treatment (24 hours) followed by 5-FU treatment (48 hours) (CT-2) and 5-FU treatment (24 hours) followed by CDDP treatment (48 hours) (CT-3). CT-1 produced the highest cytotoxicity, followed by CT-3 and CT-2. No treatment induced any detectable internucleosomal DNA fragmentation, and caspase-3,-8 and -9 were activated to a much lesser extent than that attained using actinomycin D. High-performance liquid chromatography analysis demonstrated that 5-FU, as well as CT-1 and CT-2, preferentially reduced the intracellular concentration of putrescine. These results suggest that simultaneous treatment with CDDP and 5-FU induces lower level of apoptotic cell death in HSC-2 cells. Copyright© 2007 International Institute of Anticancer Research (Dr. John G. Delinassios), All rights reserved