TY - JOUR T1 - Magnetic Resonance and Fluorescence–Protein Imaging of the Anti-angiogenic and Anti-tumor Efficacy of Selenium in an Orthotopic Model of Human Colon Cancer JF - Anticancer Research JO - Anticancer Res SP - 387 LP - 393 VL - 31 IS - 2 AU - ARUP BHATTACHARYA AU - STEVE G TUROWSKI AU - IVAN DOMINGUEZ SAN MARTIN AU - ASHWANI RAJPUT AU - YOUCEF M. RUSTUM AU - ROBERT M. HOFFMAN AU - MUKUND SESHADRI Y1 - 2011/02/01 UR - http://ar.iiarjournals.org/content/31/2/387.abstract N2 - Tumor progression and angiogenesis are intimately related. To understand the interrelationship between these two processes, real-time imaging can make a major contribution. In this report, fluorescent protein imaging (FPI) and magnetic resonance imaging (MRI) were utilized to demonstrate the effects of selenium on tumor progression and angiogenesis in an orthotopic model of human colon cancer. GEO (well-differentiated human colon carcinoma) cells transfected with green fluorescent protein (GFP) were implanted orthotopically into the colon of athymic nude mice. Beginning at five days post implantation, whole-body FPI was performed to monitor tumor growth in vivo. Upon successful visualization of tumor growth by FPI, animals were randomly assigned to either a control group or a treatment group. Treatment consisted of daily oral administration of the organoselenium compound, methyl-selenocysteine (MSC; 0.2 mg/day × five weeks). Dynamic contrast-enhanced MRI was performed to examine the change in tumor blood volume following treatment. CD31 immunostaining of tumor sections was also performed to quantify microvessel density (MVD). While T1- and T2-weighted MRI provided adequate contrast and volumetric assessment of GEO tumor growth, GFP imaging allowed for high-throughput visualization of tumor progression in vivo. Selenium treatment resulted in a significant reduction in blood volume and microvessel density of GEO tumors. A significant inhibition of tumor growth was also observed in selenium-treated animals compared to untreated control animals. Together, these results highlight the usefulness of multimodal imaging approaches to demonstrate antitumor and anti-angiogenesis efficacy and the promise of selenium treatment of colon cancer. ER -